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KMID : 0379119970250010046
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Korean Journal of Mycology
1997 Volume.25 No. 1 p.46 ~ p.67
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Taxonomic Study of Korean Armillaria Species Based on Biological Characteristics and DNA Analyses
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Harrington, Tom
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Abstract
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From 1985 to 1993, we collected 20 isolates throughout Kangwon and obtained 6 isolates from other sources. A. mellea formed rhizomorph actively, and some of A. ostoyae were poor in the formation of rhizomorph and some without formation of rhizomorph. A. tabescens was active in the growth of serial mycelium and poor in the development of rhizomorph. In A. gallica, the mycelium development among the isolates were variable greedy, and especially in isolate A8(KNU-250), the mycelial development was similar to that of A. ostoyae, but A8(KNU-250) showed the feature of A. gallica to change medium into brawn color. In PCR-RFLP analysis of the IGS region in rDNA, the homology between each isolate in the A. mellea and A. ostoyae showed 100% homology. A. tabescens showed 0.919∼0.974 homology, and A. gallica showed 0.619∼1.000 homology. A19 and A22 showed 100% homology as the same group, but compared with other subgroups they showed less than 10% homology as 0.051∼0.108 value. In RAPD analyses, the isoletes of A. mellea showed high homology among themselves as 0.983-1.000, and A. ostoyae also showed high similarity. The homology between isolates of A. tabescens showed 0.594∼0.953 value because A. gallica showed 0.280∼0:733 value, and the variations between isolates were greater than other species. Especially, A19 and A22 were identified as new novel group which were remoted from other groups, and the homology between these two isolates showed 0.921 value, and the genetic similarity between these groups and other 4 groups showed less than 7% as 0.012∼0.069 value. Of 5 species identified in this study, 4 species were identified as A. mellea, A. ostoyae, A. tabescens, and A. gallica that were already reported ones and 1 species was suggested as a new specie in Korea.
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